Current Technical Approaches to Study RNA–Protein Interactions in mRNAs and Long Non-Coding RNAs

It is commonly understood that RNA-binding proteins crucially determine the fate of their target RNAs. Vice versa, RNAs are becoming increasingly recognized for functions in protein regulation and dynamics RNA-protein complexes. Long non-coding emerging as potent regulators exert unknown properties moonlighting functions. A vast array RNA- protein-centric techniques have been developed identification RNA targets, respectively, including unbiased mass spectrometry next-generation sequencing readout. Determining true physiological targets challenging RNA–protein interaction highly dynamic, tissue- cell-type-specific, changes with environment. Here I review current analysis interactions living cells vitro. RNA-centric presented on basis cross-linking or use alternative approaches. Protein-centric approaches discussed combination high-throughput sequencing. Finally, impact mutations complexes human disease highlighted.